Genome Regulation in Mycobacterium Tuberculosis


My PhD in the lab of Eric Galburt focused on DNA-dependent processes (transcription and repair) in the pathogenic bacteria Mycobacterium tuberculosis. Using a combination of transient and steady-state kinetics, kinetic modeling, fluorescence anisotropy equilibrium titrations, and analytical ultracentrifugation (AUC), I investigated the regulation of transcription by transcription factors, small molecules, and various sigma factors. By making comparative kinetic measurements to well studied E. coli systems, I highlighted ways in which mycobacteria has diverged evolutionarily and functionally. I am currently continuing aspects of this work in the lab of Andrea Soranno, assessing protein conformational dynamics of the transcriptional machinery using single-molecule FRET.

Publications


High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymerase


Drake Jensen, Ana Ruiz Manzano, Maxwell Rector, Eric J Tomko, M. Thomas Record, Eric A. Galburt

Nucleic Acids Research, vol. 51(19), 2023


Mycobacterium tuberculosis DNA repair helicase UvrD1 is activated by redox-dependent dimerization via a 2B domain cysteine


Ankita Chadda, Drake Jensen, Eric J. Tomko, Ana Ruiz Manzano, Binh Nguyen, Timothy Lohman, Eric A. Galburt

Proceedings of the National Academy of Sciences, vol. 119(8), 2022


Molecular dissection of RbpA-mediated regulation of fidaxomicin sensitivity in mycobacteria


Jerome Prusa, Dennis X. Zhu, Aidan J. Flynn, Drake Jensen, Ana Ruiz Manzano, Eric A. Galburt, Christina L. Stallings

Journal of Biological Chemistry, vol. 298(4), 2022


CarD and RbpA modify the kinetics of initial transcription and slow promoter escape of the Mycobacterium tuberculosis RNA polymerase


Drake Jensen, Ana Ruiz Manzano, Jayan Rammohan, Christina L Stallings, Eric A Galburt

Nucleic Acids Research, vol. 47(13), 2019, pp. 6685-6698


Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets


Jerome Prusa, Drake Jensen, Gustavo Santiago-Collazo, Steven S. Pope, Ashley L. Garner, Justin J. Miller, Ana Ruiz Manzano, Eric A. Galburt, Christina L. Stallings

Journal of Bacteriology, vol. 200(13), 2018


Effects of increasing the affinity of CarD for RNA polymerase on Mycobacterium tuberculosis growth, rRNA transcription, and virulence


Ashley L. Garner, Jayan Rammohan, Jeremy P. Huynh, Lucas M. Onder, James Chen, Brian Bae, Drake Jensen, Leslie A. Weiss, Ana Ruiz Manzano, Seth A. Darst, Elizabeth A. Campbell, Bryce E. Nickels, Eric A. Galburt, Christina L. Stallings

Journal of Bacteriology, vol. 199(4), 2017


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