My PhD in the lab of Eric Galburt focused on DNA-dependent processes (transcription and repair) in the pathogenic bacteria Mycobacterium tuberculosis. Using a combination of transient and steady-state kinetics, kinetic modeling, fluorescence anisotropy equilibrium titrations, and analytical ultracentrifugation (AUC), I investigated the regulation of transcription by transcription factors, small molecules, and various sigma factors. By making comparative kinetic measurements to well studied E. coli systems, I highlighted ways in which mycobacteria has diverged evolutionarily and functionally. I am currently continuing aspects of this work in the lab of Andrea Soranno, assessing protein conformational dynamics of the transcriptional machinery using single-molecule FRET.
Publications
Drake Jensen, Ana Ruiz Manzano, Maxwell Rector, Eric J Tomko, M. Thomas Record, Eric A. Galburt
Nucleic Acids Research, vol. 51(19), 2023
Ankita Chadda, Drake Jensen, Eric J. Tomko, Ana Ruiz Manzano, Binh Nguyen, Timothy Lohman, Eric A. Galburt
Proceedings of the National Academy of Sciences, vol. 119(8), 2022
Molecular dissection of RbpA-mediated regulation of fidaxomicin sensitivity in mycobacteria
Jerome Prusa, Dennis X. Zhu, Aidan J. Flynn, Drake Jensen, Ana Ruiz Manzano, Eric A. Galburt, Christina L. Stallings
Journal of Biological Chemistry, vol. 298(4), 2022
Drake Jensen, Ana Ruiz Manzano, Jayan Rammohan, Christina L Stallings, Eric A Galburt
Nucleic Acids Research, vol. 47(13), 2019, pp. 6685-6698
Jerome Prusa, Drake Jensen, Gustavo Santiago-Collazo, Steven S. Pope, Ashley L. Garner, Justin J. Miller, Ana Ruiz Manzano, Eric A. Galburt, Christina L. Stallings
Journal of Bacteriology, vol. 200(13), 2018
Ashley L. Garner, Jayan Rammohan, Jeremy P. Huynh, Lucas M. Onder, James Chen, Brian Bae, Drake Jensen, Leslie A. Weiss, Ana Ruiz Manzano, Seth A. Darst, Elizabeth A. Campbell, Bryce E. Nickels, Eric A. Galburt, Christina L. Stallings
Journal of Bacteriology, vol. 199(4), 2017
