During my Masters at Southern Illinois University Edwardsville I taught Physical Chemistry and Biochemistry lab courses. There, I assisted in developing two teaching protocols for measuring thermodynamic binding parameters using isothermal titration calorimetry (ITC). Students in the Pchem lab explored the binding of divalent cations to EDTA and learned the importance of accounting for buffer ionization enthalpies and pH dependencies when determining observed binding enthalpies. Students in the Biochem lab explored the binding of N-Acetylglucosamine derivatives to lysozyme. This protocol included learning how to set up competition assays to measure weak binding interactions and observe hydrogen-bonding using PyMol molecular modeling.
Publications
Chin-Chuan Wei, Drake Jensen, Tiffany Boyle, Leah C. O'Brien, Cristina De Meo, Nahid Shabestary, Douglas J. Eder
Journal of Chemical Education, vol. 92(9), 2015, pp. 1552-1556
Leah C. O'Brien, Hannah B. Root, Chin-Chuan Wei, Drake Jensen, Nahid Shabestary, Cristina De Meo, Douglas J. Eder
Journal of Chemical Education, vol. 92(9), 2015, pp. 1547-1551