Calorimetry Teaching Protocols for Undergraduate Lab Courses


During my Masters at Southern Illinois University Edwardsville I taught Physical Chemistry and Biochemistry lab courses. There, I assisted in developing two teaching protocols for measuring thermodynamic binding parameters using isothermal titration calorimetry (ITC). Students in the Pchem lab explored the binding of divalent cations to EDTA and learned the importance of accounting for buffer ionization enthalpies and pH dependencies when determining observed binding enthalpies. Students in the Biochem lab explored the binding of N-Acetylglucosamine derivatives to lysozyme. This protocol included learning how to set up competition assays to measure weak binding interactions and observe hydrogen-bonding using PyMol molecular modeling.   

Publications


Isothermal Titration Calorimetry and Macromolecular Visualization for the Interaction of Lysozyme and Its Inhibitors


Chin-Chuan Wei, Drake Jensen, Tiffany Boyle, Leah C. O'Brien, Cristina De Meo, Nahid Shabestary, Douglas J. Eder

Journal of Chemical Education, vol. 92(9), 2015, pp. 1552-1556


M2+•EDTA Binding Affinities: A Modern Experiment in Thermodynamics for the Physical Chemistry Laboratory


Leah C. O'Brien, Hannah B. Root, Chin-Chuan Wei, Drake Jensen, Nahid Shabestary, Cristina De Meo, Douglas J. Eder

Journal of Chemical Education, vol. 92(9), 2015, pp. 1547-1551


Share



Follow this website


You need to create an Owlstown account to follow this website.


Sign up

Already an Owlstown member?

Log in